Рамногалактуронанска ендолијаза
| Ramnogalakturonanska endolijaza | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Identifikatori | |||||||||
| EC broj | 4.2.2.23 | ||||||||
| Baze podataka | |||||||||
| IntEnz | IntEnz pregled | ||||||||
| BRENDA | BRENDA pristup | ||||||||
| ExPASy | NiceZyme pregled | ||||||||
| KEGG | KEGG pristup | ||||||||
| MetaCyc | metabolički put | ||||||||
| PRIAM | profil | ||||||||
| Strukture PBP | RCSB PDB PDBe PDBj PDBsum | ||||||||
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Ramnogalakturonanska endolijaza (EC 4.2.2.23, hamnogalakturonaza B, alfa-L-hamnopiranozil-(1->4)-alfa-D-galaktopiranoziluronid lijaza, Rgaza B, hamnogalakturonan alfa-L-hamnopiranozil-(1,4)-alfa-D-galaktopiranoziluronid lijaza, RG-lijaza, YesW, RGL4, Rgl11A, Rgl11Y, RhiE) je enzim sa sistematskim imenom alfa-L-ramnopiranozil-(1->4)-alfa-D-galaktopiranoziluronat endolijaza.[1][2][3][4][5][6][7][8] Ovaj enzim katalizuje sledeću hemijsku reakciju
- Endotipno eliminativno razlaganje L-alfa-ramnopiranozil-(1->4)-alfa-D-galaktopiranoziluronsko kiselinskih veza ramnogalakturonan I domena pektina, tako da L-ramnopiranoza ostaje na redukujućem kraju i 4-dezoksi-4,5-nezasićena D-galaktopiranoziluronska kiselina na neredukujućem kraju
Ovaj enzim je deo degradaionog sistema ramnogalakturonana I kod Bacillus subtilis vrste 168 i Aspergillus aculeatus.
Reference
[уреди | уреди извор]- ^ Mutter, M., Colquhoun, I.J., Schols, H.A., Beldman, G. and Voragen, A.G. (1996). „Rhamnogalacturonase B from Aspergillus aculeatus is a rhamnogalacturonan α-L-rhamnopyranosyl-(1→4)-α-D-galactopyranosyluronide lyase”. Plant Physiol. 110: 73—77. PMID 8587995.
- ^ Azadi, P., O'Neill, M.A., Bergmann, C., Darvill, A.G. and Albersheim, P. (1995). „The backbone of the pectic polysaccharide rhamnogalacturonan I is cleaved by an endohydrolase and an endolyase”. Glycobiology. 5: 783—789. PMID 8720076.
- ^ Mutter, M., Colquhoun, I.J., Beldman, G., Schols, H.A., Bakx, E.J. and Voragen, A.G. (1998). „Characterization of recombinant rhamnogalacturonan α-L-rhamnopyranosyl-(1,4)-α-D-galactopyranosyluronide lyase from Aspergillus aculeatus. An enzyme that fragments rhamnogalacturonan I regions of pectin”. Plant Physiol. 117: 141—152. PMID 9576783.
- ^ Kadirvelraj, R., Harris, P., Poulsen, J.C., Kauppinen, S. and Larsen, S. (2002). „A stepwise optimization of crystals of rhamnogalacturonan lyase from Aspergillus aculeatus”. Acta Crystallogr. D Biol. Crystallogr. 58: 1346—1349. PMID 12136151.
- ^ Laatu, M. & Condemine, G. (2003). „Rhamnogalacturonate lyase RhiE is secreted by the out system in Erwinia chrysanthemi”. J. Bacteriol. 185: 1642—1649. PMID 12591882.
- ^ Pages, S., Valette, O., Abdou, L., Belaich, A. and Belaich, J.P. (2003). „A rhamnogalacturonan lyase in the Clostridium cellulolyticum cellulosome”. J. Bacteriol. 185: 4727—4733. PMID 12896991.
- ^ Ochiai, A., Yamasaki, M., Itoh, T., Mikami, B., Hashimoto, W. and Murata, K. (2006). „Crystallization and preliminary X-ray analysis of the rhamnogalacturonan lyase YesW from Bacillus subtilis strain 168, a member of polysaccharide lyase family 11”. Acta Crystallogr. Sect. F Struct. Biol. Cryst. Commun. 62: 438—440. PMID 16682770.
- ^ Jensen, M.H., Otten, H., Christensen, U., Borchert, T.V., Christensen, L.L., Larsen, S. and Leggio, L.L. (2010). „Structural and biochemical studies elucidate the mechanism of rhamnogalacturonan lyase from Aspergillus aculeatus”. J. Mol. Biol. 404: 100—111. PMID 20851126.
Literatura
[уреди | уреди извор]- Nicholas C. Price; Lewis Stevens (1999). Fundamentals of Enzymology: The Cell and Molecular Biology of Catalytic Proteins (Third изд.). USA: Oxford University Press. ISBN 019850229X.
- Eric J. Toone (2006). Advances in Enzymology and Related Areas of Molecular Biology, Protein Evolution (Volume 75 изд.). Wiley-Interscience. ISBN 0471205036.
- Branden C; Tooze J. Introduction to Protein Structure. New York, NY: Garland Publishing. ISBN 0-8153-2305-0.
- Irwin H. Segel. Enzyme Kinetics: Behavior and Analysis of Rapid Equilibrium and Steady-State Enzyme Systems (Book 44 изд.). Wiley Classics Library. ISBN 0471303097.
- William P. Jencks (1987). Catalysis in Chemistry and Enzymology. Dover Publications. ISBN 0486654605.
Spoljašnje veze
[уреди | уреди извор]- Rhamnogalacturonan+endolyase на US National Library of Medicine Medical Subject Headings (MeSH)
