23S rRNK (uridin2479-2'-O)-metiltransferaza
(преусмерено са S-adenozil-L-metionin:23S rRNK (uridin2479-2'-O)-metiltransferaza)
| 23S rRNK (uridin2479-2'-O)-metiltransferaza | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Identifikatori | |||||||||
| EC broj | 2.1.1.208 | ||||||||
| Baze podataka | |||||||||
| IntEnz | IntEnz pregled | ||||||||
| BRENDA | BRENDA pristup | ||||||||
| ExPASy | NiceZyme pregled | ||||||||
| KEGG | KEGG pristup | ||||||||
| MetaCyc | metabolički put | ||||||||
| PRIAM | profil | ||||||||
| Strukture PBP | RCSB PDB PDBe PDBj PDBsum | ||||||||
| |||||||||
23S rRNK (uridin2479-2'-O)-metiltransferaza (EC 2.1.1.208, AviRb) je enzim sa sistematskim imenom S-adenozil-L-metionin:23S rRNK (uridin2479-2'-O)-metiltransferaza.[1][2][3] Ovaj enzim katalizuje sledeću hemijsku reakciju
- S-adenozil-L-metionin + uridin2479 u 23S rRNK S-adenozil-L-homocistein + 2'-O-metiluridin2479 u 23S rRNK
Streptomyces viridochromogenes proizvodi antibiotski avilamicin A koji se vezuje za 50S ribozomalnu podjedinicu i inhibira sintezu proteina. Da bi se zaštitio od antibiotika, Streptomyces viridochromogenes koristi dve metiltransferaze, 23S rRNK (uridin2479-2'-O)-metiltransferazu i EC 2.1.1.209, 23S rRNK (guanin2535-N1)-metiltransferazu.
Reference
[уреди | уреди извор]- ^ Mosbacher, T.G., Bechthold, A. and Schulz, G.E. (2005). „Structure and function of the antibiotic resistance-mediating methyltransferase AviRb from Streptomyces viridochromogenes”. J. Mol. Biol. 345: 535—545. PMID 15581897.
- ^ Treede, I., Jakobsen, L., Kirpekar, F., Vester, B., Weitnauer, G., Bechthold, A. and Douthwaite, S. (2003). „The avilamycin resistance determinants AviRa and AviRb methylate 23S rRNA at the guanosine 2535 base and the uridine 2479 ribose”. Mol. Microbiol. 49: 309—318. PMID 12828631.
- ^ Weitnauer, G., Gaisser, S., Trefzer, A., Stockert, S., Westrich, L., Quiros, L.M., Mendez, C., Salas, J.A. and Bechthold, A. (2001). „An ATP-binding cassette transporter and two rRNA methyltransferases are involved in resistance to avilamycin in the producer organism Streptomyces viridochromogenes Tu57”. Antimicrob. Agents Chemother. 45: 690—695. PMID 11181344.
Literatura
[уреди | уреди извор]- Nicholas C. Price; Lewis Stevens (1999). Fundamentals of Enzymology: The Cell and Molecular Biology of Catalytic Proteins (Third изд.). USA: Oxford University Press. ISBN 019850229X.
- Eric J. Toone (2006). Advances in Enzymology and Related Areas of Molecular Biology, Protein Evolution (Volume 75 изд.). Wiley-Interscience. ISBN 0471205036.
- Branden C; Tooze J. Introduction to Protein Structure. New York, NY: Garland Publishing. ISBN 0-8153-2305-0.
- Irwin H. Segel. Enzyme Kinetics: Behavior and Analysis of Rapid Equilibrium and Steady-State Enzyme Systems (Book 44 изд.). Wiley Classics Library. ISBN 0471303097.
- William P. Jencks (1987). Catalysis in Chemistry and Enzymology. Dover Publications. ISBN 0486654605.
Spoljašnje veze
[уреди | уреди извор]- 23S+rRNA+(uridine2479-2'-O)-methyltransferase на US National Library of Medicine Medical Subject Headings (MeSH)
