Метилентетрахидрофолат—тРНК-(урацил-5-)-метилтрансфераза
| Metilentetrahidrofolat—tRNK-(uracil-5-)-metiltransferaza | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Identifikatori | |||||||||
| EC broj | 2.1.1.74 | ||||||||
| CAS broj | 56831-74-4 | ||||||||
| Baze podataka | |||||||||
| IntEnz | IntEnz pregled | ||||||||
| BRENDA | BRENDA pristup | ||||||||
| ExPASy | NiceZyme pregled | ||||||||
| KEGG | KEGG pristup | ||||||||
| MetaCyc | metabolički put | ||||||||
| PRIAM | profil | ||||||||
| Strukture PBP | RCSB PDB PDBe PDBj PDBsum | ||||||||
| |||||||||
Metilentetrahidrofolat—tRNK-(uracil-5-)-metiltransferaza (EC 2.1.1.74, folat-zavisna ribotimidilna sintaza, metilintetrahidrofolatni-transfer ribonukleatni uracil 5-metiltransferaza, 5,10-metilintetrahidrofolat:tRNK-UPsiC (uracil-5-)-metil-transferaza, 5,10-metilintetrahidrofolat:tRNK (uracil-5-)-metil-transferaza, TrmFO, folat/FAD-zavisni tRNK T54 metiltransferaza) je enzim sa sistematskim imenom 5,10-metilintetrahidrofolat:tRNK (uracil54-C5)-metiltransferaza.[1][2][3] Ovaj enzim katalizuje sledeću hemijsku reakciju
- 5,10-metilintetrahidrofolat + uridin54 unutar tRNK + FADH2 tetrahidrofolat + 5-metiluridin54 unutar tRNK + FAD
Do 25% baza funkcionalne tRNK je posttranslaciono modifikovano ili hipermodifikovano. Jedna od skoro universalnih posttranslacionih modifikacija je konverzija U54 u ribotimidinu u TPsiC petlji.
Reference
[уреди | уреди извор]- ^ Delk, A.S., Nagle, D.P., Jr. and Rabinowitz, J.C. (1980). „Methylenetetrahydrofolate-dependent biosynthesis of ribothymidine in transfer RNA of Streptococcus faecalis. Evidence for reduction of the 1-carbon unit by FADH2”. J. Biol. Chem. 255: 4387—4390. PMID 6768721.
- ^ Becker, H.F., Motorin, Y., Sissler, M., Florentz, C. and Grosjean, H. (1997). „Major identity determinants for enzymatic formation of ribothymidine and pseudouridine in the TΨ-loop of yeast tRNAs”. J. Mol. Biol. 274: 505—518. PMID 9417931.
- ^ Nishimasu, H., Ishitani, R., Yamashita, K., Iwashita, C., Hirata, A., Hori, H. and Nureki, O. (2009). „Atomic structure of a folate/FAD-dependent tRNA T54 methyltransferase”. Proc. Natl. Acad. Sci. USA. 106: 8180—8185. PMID 19416846.
Literatura
[уреди | уреди извор]- Nicholas C. Price; Lewis Stevens (1999). Fundamentals of Enzymology: The Cell and Molecular Biology of Catalytic Proteins (Third изд.). USA: Oxford University Press. ISBN 019850229X.
- Eric J. Toone (2006). Advances in Enzymology and Related Areas of Molecular Biology, Protein Evolution (Volume 75 изд.). Wiley-Interscience. ISBN 0471205036.
- Branden C; Tooze J. Introduction to Protein Structure. New York, NY: Garland Publishing. ISBN 0-8153-2305-0.
- Irwin H. Segel. Enzyme Kinetics: Behavior and Analysis of Rapid Equilibrium and Steady-State Enzyme Systems (Book 44 изд.). Wiley Classics Library. ISBN 0471303097.
Spoljašnje veze
[уреди | уреди извор]- Methylenetetrahydrofolate—tRNA-(uracil54-C5)-methyltransferase+(FADH2-oxidizing) на US National Library of Medicine Medical Subject Headings (MeSH)
