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Holestanetriol 26-monooksigenaza

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Holestanetriol 26-monooksigenaza
Identifikatori
EC broj1.14.13.15
CAS broj52227-77-7
Baze podataka
IntEnzIntEnz pregled
BRENDABRENDA pristup
ExPASyNiceZyme pregled
KEGGKEGG pristup
MetaCycmetabolički put
PRIAMprofil
Strukture PBPRCSB PDB PDBe PDBj PDBsum

Holestanetriol 26-monooksigenaza (EC 1.14.13.15, 5beta-holestan-3alfa,7alfa,12alfa-triol 26-hidroksilaza, 5beta-holestan-3alfa,7alfa,12alfa-triol hidroksilaza, holestantriolna 26-hidroksilaza, sterolna 27-hidroksilaza, sterolna 26-hidroksilaza, holesterolna 27-hidroksilaza, CYP27A, CYP27A1, citohrom P450 27A1) je enzim sa sistematskim imenom 5beta-holestan-3alfa,7alfa,12alfa-triol,NADPH:kiseonik oksidoreduktaza (26-hidroksilacija).[1][2][3][4][5][6][7][8][9] Ovaj enzim katalizuje sledeću hemijsku reakciju

5beta-holestan-3alfa,7alfa,12alfa-triol + 3 NADPH + 3 H+ + 3 O2 (25R)-3alfa,7alfa,12alfa-trihidroksi-5beta-holestan-26-oat + 3 NADP+ + 4H2O (sveukupna reakcija)
(1a) 5beta-holestan-3alfa,7alfa,12alfa-triol + NADPH + H+ + O2 (25R)-5beta-holestan-3alfa,7alfa,12alfa,26-tetraol + NADP+ +H2O
(1b) (25R)-5beta-holestan-3alfa,7alfa,12alfa,26-tetraol + NADPH + H+ + O2 (25R)-3alfa,7alfa,12alfa-trihidroksi-5beta-holestan-26-al + NADP+ + 2H2O
(1c) (25R)-3alfa,7alfa,12alfa-trihidroksi-5beta-holestan-26-al + NADPH+ + H+ + O2 (25R)-3alfa,7alfa,12alfa-trihidroksi-5beta-holestan-26-oat + NADP+ +H2O

Za rad ovog enzima su neophodni feredoksin i feredoksinska reduktaza.

Reference

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  1. ^ Masui, T., Herman, R. and Staple, E. (1966). „The oxidation of 5β-cholestane-3α,7α,12α,26-tetraol to 5β-cholestane-3α,7α,12α-triol-26-oic acid via 5β-cholestane-3α,7α,12α-triol-26-al by rat liver”. Biochim. Biophys. Acta. 117: 266—268. PMID 5914340. 
  2. ^ Okuda, K. & Hoshita, N. (1968). „Oxidation of 5β-cholestane-3α,7α,12α-triol by rat-liver mitochondria”. Biochim. Biophys. Acta. 164: 381—388. PMID 4388637. 
  3. ^ Wikvall, K. (1984). „Hydroxylations in biosynthesis of bile acids. Isolation of a cytochrome P-450 from rabbit liver mitochondria catalyzing 26-hydroxylation of C27-steroids”. J. Biol. Chem. 259: 3800—3804. PMID 6423637. 
  4. ^ Andersson, S., Davis, D.L., Dahlbäck, H., Jörnvall, H. and Russell, D.W. (1989). „Cloning, structure, and expression of the mitochondrial cytochrome P-450 sterol 26-hydroxylase, a bile acid biosynthetic enzyme”. J. Biol. Chem. 264: 8222—8229. PMID 2722778. 
  5. ^ Dahlback, H. & Holmberg, I. (1990). „Oxidation of 5β-cholestane-3α,7α,12α-triol into 3α,7α,12α-trihydroxy-5β-cholestanoic acid by cytochrome P-45026 from rabbit liver mitochondria”. Biochem. Biophys. Res. Commun. 167: 391—395. PMID 2322231. 
  6. ^ Holmberg-Betsholtz, I., Lund, E., Björkhem, I. and Wikvall, K. (1993). „Sterol 27-hydroxylase in bile acid biosynthesis. Mechanism of oxidation of 5β-cholestane-3α,7α,12α,27-tetrol into 3α,7α,12α-trihydroxy-5β-cholestanoic acid”. J. Biol. Chem. 268: 11079—11085. PMID 8496170. 
  7. ^ Pikuleva, I.A., Babiker, A., Waterman, M.R. and Bjorkhem, I. (1998). „Activities of recombinant human cytochrome P450c27 (CYP27) which produce intermediates of alternative bile acid biosynthetic pathways”. J. Biol. Chem. 273: 18153—18160. PMID 9660774. 
  8. ^ Furster, C., Bergman, T. and Wikvall, K. (1999). „Biochemical characterization of a truncated form of CYP27A purified from rabbit liver mitochondria”. Biochem. Biophys. Res. Commun. 263: 663—666. PMID 10512735. 
  9. ^ Pikuleva, I.A., Puchkaev, A. and Björkhem, I. (2001). „Putative helix F contributes to regioselectivity of hydroxylation in mitochondrial cytochrome P450 27A1”. Biochemistry. 40: 7621—7629. PMID 11412116. 

Literatura

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Spoljašnje veze

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