Holestenon 5a-reduktaza
| Holestenon 5a-reduktaza | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Identifikatori | |||||||||
| EC broj | 1.3.1.22 | ||||||||
| CAS broj | 37255-34-8 | ||||||||
| Baze podataka | |||||||||
| IntEnz | IntEnz pregled | ||||||||
| BRENDA | BRENDA pristup | ||||||||
| ExPASy | NiceZyme pregled | ||||||||
| KEGG | KEGG pristup | ||||||||
| MetaCyc | metabolički put | ||||||||
| PRIAM | profil | ||||||||
| Strukture PBP | RCSB PDB PDBe PDBj PDBsum | ||||||||
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Holestenon 5a-reduktaza (EC 1.3.1.22, holestenonska 5alfa-reduktaza, testosteronska Delta4-5alfa-reduktaza, steroidna 5alfa-reduktaza, 3-oksosteroidna Delta4-dehidrogenaza, 5alfa-reduktaza, steroidna 5alfa-hidrogenaza, 3-oksosteroidna 5alfa-reduktaza, testosteronska Delta4-hidrogenaza, 4-en-3-oksosteroidna 5alfa-reduktaza, redukovani nikotinamid adenin dinukleotid fosfat:Delta4-3-ketosteroid 5alfa-oksidoreduktaza, 4-en-5alfa-reduktaza, Delta4-3-ketosteroid 5alfa-oksidoreduktaza, holest-4-en-3-on 5alfa-reduktaza, testosteronska 5alfa-reduktaza, 3-okso-5alfa-steroidna 4-dehidrogenaza) je enzim sa sistematskim imenom 3-okso-5alfa-steroid:NADP+ Delta4-oksidoreduktaza.[1][2][3][4][5][6][7][8] Ovaj enzim katalizuje sledeću hemijsku reakciju
- 3-okso-5alfa-steroid + NADP+ 3-okso-Delta4-steroid + NADPH + H+
Ovaj enzim katalizuje konverziju pojedinih 3-okso-Delta4 steroida u njihovu korespondirajuću 5 alfa formu. Supstrati sisarskog enzima su testosteron, progesteron, i kortikosteron. Supstrati biljnog enzima su brasinosteroidi kao što je kampest-4-en-3-on i (22 alfa)-hidroksi-kampest-4-en-3-on, cf. EC 1.3.99.5, 3-okso-5alfa-steroid 4-dehidrogenaza (akceptor).
Reference
[уреди | уреди извор]- ^ Levy, H.R. & Talalay, P. (1959). „Bacterial oxidation of steroids. II. Studies on the enzymatic mechanisms of ring A dehydrogenation”. J. Biol. Chem. 234: 2014—2021. PMID 13673006.
- ^ Shefer, S., Hauser, S. and Mosbach, E.H. (1966). „Studies on the biosynthesis of 5α-cholestan-3β-ol. I. Cholestenone 5α-reductase of rat liver”. J. Biol. Chem. 241: 946—952. PMID 5907469.
- ^ Cheng, Y.-J. & Karavolas, H.J. (1975). „Properties and subcellular distribution of Δ4-steroid (progesterone) 5α-reductase in rat anterior pituitary”. Steroids. 26: 57—71. PMID 1166484.
- ^ Sargent, N.S. & Habib, F.K. (1991). „Partial purification of human prostatic 5α-reductase (3-oxo-5α-steroid:NADP+ 4-ene-oxido-reductase; EC 1.3.1.22) in a stable and active form”. J. Steroid Biochem. Mol. Biol. 38: 73—77. PMID 1705142.
- ^ Quemener, E., Amet, Y., di Stefano, S., Fournier, G., Floch, H.H. and Abalain, J.H. (1994). „Purification of testosterone 5α-reductase from human prostate by a four-step chromatographic procedure”. Steroids. 59: 712—718. PMID 7900170.
- ^ Poletti, A., Celotti, F., Rumio, C., Rabuffetti, M. and Martini, L. (1997). „Identification of type 1 5α-reductase in myelin membranes of male and female rat brain”. Mol. Cell. Endocrinol. 129: 181—190. PMID 9202401.
- ^ Li, J., Biswas, M.G., Chao, A., Russell, D.W. and Chory, J. (1997). „Conservation of function between mammalian and plant steroid 5α-reductases”. Proc. Natl. Acad. Sci. USA. 94: 3554—3559. PMID 9108014.
- ^ Rosati, F., Bardazzi, I., De Blasi, P., Simi, L., Scarpi, D., Guarna, A., Serio, M., Racchi, M.L. and Danza, G. (2005). „5α-Reductase activity in Lycopersicon esculentum: cloning and functional characterization of LeDET2 and evidence of the presence of two isoenzymes”. J. Steroid Biochem. Mol. Biol. 96: 287—299. PMID 15993049.
Literatura
[уреди | уреди извор]- Nicholas C. Price; Lewis Stevens (1999). Fundamentals of Enzymology: The Cell and Molecular Biology of Catalytic Proteins (Third изд.). USA: Oxford University Press. ISBN 019850229X.
- Eric J. Toone (2006). Advances in Enzymology and Related Areas of Molecular Biology, Protein Evolution (Volume 75 изд.). Wiley-Interscience. ISBN 0471205036.
- Branden C; Tooze J. Introduction to Protein Structure. New York, NY: Garland Publishing. ISBN 0-8153-2305-0.
- Irwin H. Segel. Enzyme Kinetics: Behavior and Analysis of Rapid Equilibrium and Steady-State Enzyme Systems (Book 44 изд.). Wiley Classics Library. ISBN 0471303097.
- William P. Jencks (1987). Catalysis in Chemistry and Enzymology. Dover Publications. ISBN 0486654605.
Spoljašnje veze
[уреди | уреди извор]- Cholestenone+5alpha-reductase на US National Library of Medicine Medical Subject Headings (MeSH)
